Decreased Gap Width in a Cylindrical High-Field Asymmetric Waveform Ion Mobility Spectrometry Device Improves Protein Discovery
نویسندگان
چکیده
منابع مشابه
Miniaturized ultra high field asymmetric waveform ion mobility spectrometry combined with mass spectrometry for peptide analysis.
Miniaturized ultra high field asymmetric waveform ion mobility spectrometry (ultra-FAIMS) combined with mass spectrometry (MS) has been applied to the analysis of standard and tryptic peptides, derived from α-1-acid glycoprotein, using electrospray and nanoelectrospray ion sources. Singly and multiply charged peptide ions were separated in the gas phase using ultra-FAIMS and detected by ion tra...
متن کاملSeparation and Identification of Isomeric Glycopeptides by High Field Asymmetric Waveform Ion Mobility Spectrometry
The analysis of intact glycopeptides by mass spectrometry is challenging due to the numerous possibilities for isomerization, both within the attached glycan and the location of the modification on the peptide backbone. Here, we demonstrate that high field asymmetric wave ion mobility spectrometry (FAIMS), also known as differential ion mobility, is able to separate isomeric O-linked glycopepti...
متن کاملHigh-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans.
High-field asymmetric waveform ion mobility spectrometry (FAIMS) is shown to be capable of resolving isomeric and isobaric glycosaminoglycan negative ions and to have great utility for the analysis of this class of molecules when combined with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and tandem mass spectrometry. Electron detachment dissociation (EDD) and other ion...
متن کاملResolution enhancement of field asymmetric waveform ion mobility spectrometry (FAIMS) by ion focusing
BACKGROUND Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) is a material analysis technology which develops very fast in recent years. Resolution is an important factor used to estimate the performance of this technology. With greater resolution, it's always easier to separate complex mixtures. RESULTS A method to increase the resolution of FAIMS is put forward which focuses ions ...
متن کاملDiscrimination of combustion fuel sources using gas chromatography-planar field asymmetric-waveform ion mobility spectrometry
a) Department of Chemistry and Biochemistry ([email protected]), New Mexico State University, Las Cruces, NM 88003 USDA-ARS-SW Cotton Ginning Research Lab ([email protected]), 300 E. College Drive, PO Box 578, Mesilla Park, NM 88047 c) SIONEX Corporation ([email protected]), 300 Second Ave., Waltham, MA 02451 Discrimination of combustion fuel sources using gas chromatography-planar field asymmetr...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2015
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.5b03199